eBioscience Featured Cytokines:
Featured Mouse Cytokine Products - IL-13, IL-17A, IL-23
|
|
|
|
|
|
Mouse IL-13 (clone eBio13A): BALB/c splenocytes were cultured for 3 days with plate-bound anti-mouse CD3e (clone 145-2C11, cat. 16-0031), soluble anti-mouse CD28 (clone 37.51, cat. 16-0281), recombinant mouse IL-2 (cat. 14-8021), and recombinant mouse IL-4 (cat. 14-8041). Cells were then harvested and restimulated with PMA/Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with FITC anti-mouse CD4 (clone RM4-5, cat. 11-0042), fixed and permeabilized, then stained with 0.06μg Alexa Fluor® 647 eBio13A (left). The ligand blocking control demonstrates that pre-blocking the Alexa Fluor® 647 conjugated eBio13A antibody with 1μg of recombinant mouse IL-13 (cat. 14-8131) prior to staining abrogates specific mouse IL-13 staining (right).
|
|
|
Click here for more information regarding Mouse IL-13.
|
|
|
|
|
|
Mouse IL-17A (clone eBio17B7): EL-4 cells were stimulated overnight with PMA/Ionomycin in the present of monensin. Cells were harvested, fixed, permeabilized, and then intracellularlly stained with Alexa Fluor® 647 eBio17B7 (left). Co-staining eBioTC11-18H10 Alexa Fluor® 488 and eBio17B7 Alexa Fluor® 647 (right).
|
|
|
Click here for more information regarding Mouse IL-17A.
Click here for more information regarding Mouse IL-23.
